南华大学学报：自然科学版2011，Vol.25Issue(4)：105-108,4.
枸骨根中三萜类物质含量的测定
Determination of Triterpenes from the Root of Ilex Cornuta
王慧 1沈鹏飞 2曾荣今1
作者信息
- 1. 湖南科技大学理论化学与分子模拟教育部部省共建重点实验室,湖南湘潭411201／湖南科技大学化学化工学院,湖南湘潭411201
- 2. 湖南科技大学化学化工学院,湖南湘潭411201
- 折叠
摘要
Abstract
The total triterpenoids in the root of Ilex cornuta was quantified by spectrometry method using ursolic acid as standard and 5% vanillin-glacial acetic acid and perchloric acid as color-developing agents.The results showed that the content of the standard was well correlated to its optical density at 549 nm,the calibration curve was linear（R2=0.9984）in the range of 4.4~ 26.4 mg/L within 5~40 min and the curve was y=0.0357x-0.0809.The above method for the quantification was proved to have the advantages of simple operation,rapidity,high sensitivity and fine repetition.关键词
枸骨根/总三萜/分光光度法/熊果酸Key words
the root of Ilex cornuta/total triterpenes/spectrophotometry/ursolic acid分类
化学化工引用本文复制引用
王慧,沈鹏飞,曾荣今..枸骨根中三萜类物质含量的测定[J].南华大学学报：自然科学版,2011,25(4):105-108,4.
