南京工业大学学报（自然科学版）2011，Vol.33Issue(6)：102-106,5.DOI:10.3969/j.issn.1671-7627.2011.06.021
单价流感疫苗脂质体干粉细胞免疫研究
Cellular immunity of monovalent influenza vaccine lyophilized liposome
摘要
Abstract
To evaluate the immunogenicity of the influenza vaccine lyophilized liposomes through cellular immunity, it could be confirm that, with the increasing of the stability, the immunogenicity of the influenza vaccine lyophilized liposomes by pulmonary delivery was better than that of the influenza vaccine non-liposome immunized by intraperitoneal injection. Experimental mice were divided into two groups, and each group was divided into the negative control group, the influenza vaccine lyophilized liposome group, the influenza vaccine non-liposome group, and the positive control group (n = 5). The influenza vaccine lyophilized liposome group and the influenza vaccine non-liposome group were immunized with 6 |xg hemagglutinin of H1N1 per mouse through pulmonary deli very, and the positive control group was immunized with 6 u,g hemagglutinin of H1N1 per mouse through intraperitoneal injection. MTT method was used to measure the spleen cell proliferation after immunization to mice 14 days and 28 days in order to study the immunogenicity of the influenza vaccine lyophilized liposome. The influenza vaccine lyophilized liposome could induce the cellular immunity of mice by pulmonary delivery and the level of the cellular immunity was higher than that of the influenza vaccine non-liposome immunized through intraperitoneal injection. Pulmonary delivery of the influenza vaccine lyophilized liposome could achieve satisfied cellular immunity effect.关键词
流感疫苗脂质体/肺部给药/免疫原性/MTT/细胞免疫Key words
influenza vaccine lyophilized liposome/pulmonary delivery/immunogenicity/MTT/cellularimmunity分类
医药卫生引用本文复制引用
刘洁,马波,鲁卫东,徐勇军,林华,代云波..单价流感疫苗脂质体干粉细胞免疫研究[J].南京工业大学学报（自然科学版）,2011,33(6):102-106,5.基金项目
云南省科技厅应用基础研究面上项目(2008ZC113M) （2008ZC113M）
