南京林业大学学报（自然科学版）2011，Vol.35Issue(6)：29-33,5.DOI:10.3969/j.issn.1000-2006.2011.06.006
银杏种仁中银杏酚酸的提取及组分研究
Study on extraction and compositions of ginkgolic acids in ginkgo seeds
摘要
Abstract
Ginkgolic acids were the allergen in Ginkgo seeds. Based on the results of single factor experiments, the orthogonal experiment were designed to optimize extraction conditions, and the compositions of ginkgolic acids in ginkgo seeds were detected by HPLC. The result indicated that the effect of factors on the extraction was in the order as follows, ethanol concentration, the most important one, and then ratio of material to liquid and extracting time. The optimum conditions defined at last were ethanol concentration 85 % , extraction time 12 h, ratio of material to liquid 1:12.5. At this conditions, five ginkgolic acid monomers were detected in accordance with standard samples. Verification experiment suggested the extraction yield of ginkgolic acids reached 147. 94 μg/g in dry weight, and the residual rate of ginkgolic acids was 2. 19 % which was significant lower than the international standard. This method could offer a technological support for the detection of ginkgolic acids and quality control of ginkgo products.关键词
银杏种仁/银杏酚酸/提取/组分Key words
ginkgo seeds/ ginkgolic acids/ extract/ compositions分类
农业科技引用本文复制引用
潘红梅,吴彩娥,曹福亮,范龚健,杨剑婷,贾韶千,李婷婷..银杏种仁中银杏酚酸的提取及组分研究[J].南京林业大学学报（自然科学版）,2011,35(6):29-33,5.基金项目
教育部博士点基金资助项目(20080298004) （20080298004）
江苏高校优势学科建设工程资助项目 （）
国家林业局林业公益性行业科研专项项目(201004015) （201004015）
