南京农业大学学报2011，Vol.34Issue(6)：20-24,5.
影响青花菜游离小孢子培养的因素
The factors affecting isolated microspore culture in Brassica oleracea L.var.italica
摘要
Abstract
This research selected ten different types of Brassica oleracea L. Var. Italica as test materials, and studied the genotype types,childhood microspores period of cytology observation,culture medium type and pH value etc in isolated microspore culture system of Brassica oleracea L. Var. Italica. The test results showed that, donor plant genotype was the main factor for Brassica oleracea L. Var. Italica microspore embryo thallose yield, and the embryonic induction frequency was difference in different varieties. The optimal length of buds in different genotypes also had certain differences. There was a relationship between the buds and the cytological stage of microspores in Brassica oleracea L. Var. Italica and the suitable of buds had difference in different genotypes. 1/2 NLN+130 g sugar was the best culture medium of the isolated microspore; the best of the culture medium pH value was 6. 2. The suitable incubation time of the cotyledons embryos in liquid medium was 25 days. In MS medium under the appropriate agarose concentration(7.0g·L-1 ) condition the survival rates of embryo thallose seedlings was highest.关键词
青花菜/游离小孢子培养/胚状体/植株再生Key words
Brassica oleracea L. Var. Italica/isolated microspore culture/embryos thallose/plant regeneration分类
农业科技引用本文复制引用
冯翠,曾爱松,严继勇,宋立晓,高兵,侯喜林..影响青花菜游离小孢子培养的因素[J].南京农业大学学报,2011,34(6):20-24,5.基金项目
"十一五"国家科技支撑计划项目(2006BAD01 A7,2006BAD13B06) （2006BAD01 A7,2006BAD13B06）
江苏省农业科技自主创新资金项目(CX(09)604) （CX(09）
