南京农业大学学报2011，Vol.34Issue(6)：31-35,5.
外源24-表油菜素内酯对低氧胁迫下黄瓜幼苗生长和可溶性蛋白表达的影响
Effects of exogenous 24-epibrassinolide on the growth and soluble protein expression in cucumber seedlings under hypoxia stress
摘要
Abstract
Exogenous 24-epibrassinolide ( EBR ) on growth, the soluble protein content and expression was conducted with hypoxia-sensitive cucumber( Cucumis sativus L. ) ' Zhongnong 6' by hydroponic culture under hypoxia stress. Results showed that hypoxia stress significantly decreased the cucumber growth and soluble protein content in roots,but increased that in leaves. Exogenous EBR inhibited the changes of growth and soluble protein content under hypoxia stress. Hypoxia stress induced a new protein in cucumber roots and increased the abundance of eleven proteins at least, with relative molecular weight ( x 103) such as 89. 95,82. 53,66. 21, 60.42,56.17,38.44,34.71,33.25,30.21,28.03 and 18. 24. However,exogenous EBR could decrease significantly the expression of these proteins and markedly affected nine protein bands in leaves,with relative molecular weight( ×103)such as 109.68,68.38, 52.27,47.43,41.73,40.52,39.33,33.94 and 14.40. Therefore,it was possible that these proteins had relations with exogenous EBR alleviating the effect of hypoxia stress in cucumber seedlings. Thus,we considered exogenous EBR could alleviat the inhibition of hypoxia stress on cucumber seedlings by changing the soluble protein content and expression.关键词
黄瓜/低氧胁迫/24-表油菜素内酯/可溶性蛋白/SDS-PAGEKey words
cucumber/hypoxia stress/24-epibrassinolide(EBR)/soluble protein/SDS-PAGE分类
农业科技引用本文复制引用
陆晓民,孙锦,郭世荣,何立中..外源24-表油菜素内酯对低氧胁迫下黄瓜幼苗生长和可溶性蛋白表达的影响[J].南京农业大学学报,2011,34(6):31-35,5.基金项目
国家自然科学基金项目(30871736) （30871736）
国家重点基础研究发展计划项目(2009CB119000) （2009CB119000）
现代农业产业技术体系建设专项(CARS-25-C-03) （CARS-25-C-03）
江苏省农业三项工程资助项目(SX(2010)087) （SX(2010）
