南京农业大学学报2011，Vol.34Issue(6)：77-81,5.
利用转座子TnYLB-1构建枯草芽孢杆菌的突变体文库
Construction of transposition insertion library of Bacillus subtilis by using transposon TnYLB-1
摘要
Abstract
In order to identify new genes involved in colonizing in plant rhizosphere and promoting plant growth of Bacillus subtilis,in-sertional mutagenesis of B. Subtilis OKB105 was performed by use of a Himarl -based transposon TnYLB-1. The plasmid pMarA carrying the transposon TnYLB-1 were transformed into OKB105 and 2 000 transposants were selected randomly under the condition of high temperature. PCR and Southern hybridization analyses of 15 transposants showed that TnYLB-1 transposed into the B. Subtilis chromosome randomly.关键词
枯草芽孢杆菌OKB105/TnYLB-1转座子/pMarA质粒/Himar1转座元件/随机诱变Key words
Bacillus subtilis OKB105/ transposon TnYLB-1/ plasmid pMarA/transposable element Himarl/random mutagenesis分类
农业科技引用本文复制引用
马欣,刘俊,乔俊卿,伍辉军,高学文..利用转座子TnYLB-1构建枯草芽孢杆菌的突变体文库[J].南京农业大学学报,2011,34(6):77-81,5.基金项目
国家转基因生物新品种培育重大专项(2011ZX08004-004) （2011ZX08004-004）
国际科技合作项目(2009DFA32740) （2009DFA32740）
公益性行业(农业)科研专项(3-23) （农业）
国家863计划项目(2006AA10Z172) （2006AA10Z172）
国家大学生创新性实验计划项目(091030707) （091030707）
