南京医科大学学报（自然科学版）2011，Vol.31Issue(11)：1543-1549,7.
Sublytic C5b-9诱导肾小球系膜细胞产生的TSP-1和TGF-β1对其合成FN和collagen Ⅳ的影响
The effects of TSP-1 and TGF-β1 induced by sublytic C5b-9 on FN and collagen IV synthesis from glomerular mesangial cell
摘要
Abstract
Objective; To explore the roles of thrombospondin-1 (TSP-1) and transforming growth factor-β1 (TGF-β1) in mediat-ing the secretion of extracellular matrix (ECM) ,such as fibronectin (FN) and collagen Ⅳ,from rat glomerular mesangia] cell (GMC) induced by sublytic C5b-9 complexes. Methods; Rat GMC was cultured and divided into different groups. The expression levels of TSP-1 and TGF-β1 as well as FN and collagen Ⅳ in GMC with sublytic C5b-9 stimulation were measured. In addition,the synthetic blocking peptide of TSP-1 (GGWSHW) and TGF-β1 neutralizing antibody were utilized to explore the role of TSP-1 and TGF-β1 in ECM secretion from CMC induced by sublytic C5b-9,as well as the relationship between TSP-1 and TGF-β1. Results; The protein levels of FN and collagen Ⅳ as well as TSP-1 and TGF-β1 including the activated TGF-β1 were significantly increased in rat GMC at 18 h after sublytic C5b-9 stimulation. The blocking peptides of TSP-1 (GGWSHW) could significantly inhibit TGF-β1 activation and reduce the production of FN and collagen Ⅳ. In addition,TGF-β1 neutralizing antibody could also obviously inhibit the expression of FN and collagen Ⅳ in GMC upon sublytic C5b-9 attack. Conclusion: Sublytic C5b-9 can promote ECM secretion and TSP-1 and TGF-pi production as well as TGF-β1 activation in rat GMC in vitro,while the secretion of ECM stimulated by sublytic C5b-9 is possibly mediated in part by TSP-1-regulated TGF-PI activation.关键词
sublytic C5b-9/肾小球系膜细胞/血小板反应蛋白-1/转化生长因子-β1/纤维连接蛋白/Ⅳ型胶原蛋白Key words
sublytic CSb-9/glomerular mesan gial cell/ thrombospondin-1/transforming growth factor-β1/fibronectin/col-lagen Ⅳ分类
医药卫生引用本文复制引用
邱文,车楠,周建博,李妍,赵聃,王迎伟..Sublytic C5b-9诱导肾小球系膜细胞产生的TSP-1和TGF-β1对其合成FN和collagen Ⅳ的影响[J].南京医科大学学报（自然科学版）,2011,31(11):1543-1549,7.基金项目
国家自然科学基金资助(31000396,81072402) （31000396,81072402）
江苏省高校自然科学研究(10KJB310006) （10KJB310006）
南京医科大学科技发展基金面上项目(09NJMUM003) （09NJMUM003）
南京医科大学基础医学院青年教师培养基金(09JC007) （09JC007）
