南京医科大学学报（自然科学版）2011，Vol.31Issue(11)：1587-1592,6.
pEGFP-N1/Arf6-T27N重组真核表达载体的构建及其在人乳腺癌细胞株中的表达
Construction of the recombinant eukaryote expression vector carrying encoding gene of Arf6-T27N and its expression in breast cancer cells
摘要
Abstract
Objective: To construct the recombinant eukaryote expression vector containing Arf6-T27N gene and detect the protein expression in its target cell line,MDA-MB-231. Methods: The fragment of Arf6-T27N gene from expression vector pXS-Arf6-T27N was cloned into the eukaryote expression vector pEGFP-N1.then the recombinant vector pEGFP-Nl/Arf6-T27N were transfected into the MDA-MB-231 cells. After transfection of the recombinant vector, the protein expression of Arf6-T27N in MDA-MB-231 cells were detected by Western blot The effect of Arf6-T27N on cell migration was investigated by Boyden chamber assay. Results; The recombinant eukaryote expression vector carrying Arf6-T27N was constructed successfully. The expression of Arf6-T27N in MDA-MB-231 cells could be detected by Western blot In addition,Arf6-T27N protein could inhibit the migration of MDA-MB-231 cells. Conclusion: The new recombinant expression vector pEGFP-N1/Arf6-T27N was constructed successfully and the MDA-MB-231 cells which could express Arf6-T27N stably were also successfully made. Furthermore,our study suggested that Arf6-T27N protein could inhibit the migration of MDA-MB-231 cells.关键词
Arf6-T27N/MDA-MB-231人乳腺癌细胞/基因转染/稳定表达/细胞迁移Key words
Arf6-T27N/ MDA-MB-231 breast cancer cell/gene transfection/stable expression/cell migration分类
生物科学引用本文复制引用
李卫星,杜军,胡圳圳..pEGFP-N1/Arf6-T27N重组真核表达载体的构建及其在人乳腺癌细胞株中的表达[J].南京医科大学学报（自然科学版）,2011,31(11):1587-1592,6.基金项目
南京医科大学基础医学院青年教师培养基金(2010) （2010）
南京医科大学科技发展基金重点项目(2010NJ-MUZ35) （2010NJ-MUZ35）
中国博士后科学基金(2011 M501254) （2011 M501254）
