南京医科大学学报（自然科学版）2012，Vol.32Issue(1)：20-25,6.
OX40/FOX40L逆向信号对小鼠主动脉内皮细胞MMP-9表达及机制的研究
The effects and mechanism of OX40/OX40L reverse signaling on the expression of MMP9 in mouse aortic endothelial cells
摘要
Abstract
Objective; To investigate the effects of 0X40 ligand(OX40L) reverse signaling on the expression and activity of matrix metalloproteinase-9 (MMP-9) in the cultured mouse aortic endothelial cells (MAECs). Methods: Aortic endothelial cells were isolated from C57BL/6J mice aged 8 to 10 weeks and cultured to the second passage for experiments. The cultured MAECs were divided into 4 groups:control group,sOX40 group, sOX40+anti-OX40L group and sOX40+Bapta-AM group,and stimulated with DMEM,soluble OX40,anti-OX40L monoclonal antibody and calcium chelator Bapta-AM,respectively. The mRNA and protein expressions of MMP-9 were determined by RT-PCR and Western blot. The activity of MMP-9 was assayed by zymography. The intracellular calcium ([Ca2+]) in MAECs was detected by calcium imaging using fura-2. Results; A significant increase in intracellular calcium level of MAECs was observed after stimulation with soluble OX40 (0.1 mg/L) for 48 hours compared to the control,and MMP-9 mRNA and protein levels were also markedly elevated. The activity changes of MMP-9 in cell culture supernatant was consistent with that of intracellular level. Notably,these elevations can be blocked by soluble anti-OX40L monoclonal antibody (0.1 mg/L) or Bapta-AM(20 μmod/L). Conclusion:The stimulation of OX40L reverse signaling can activate the expression of MMP-9 on both mRNA and protein levels through the elevation of intracellular calcium of MAECs,suggesting that the OX40/OX40L signaling pathway may be one of the pathways contributing to the MMP-9 elevation in progression of atherosclerosis.关键词
OX40配体/钙离子/基质金属蛋白酶-9/内皮细胞Key words
OX40 ligand/ [Ca2+]/ matrix metallopro-teinase-9/ endothelial cells分类
生物科学引用本文复制引用
张丹丹,夏文龙,刘强,张大伟,徐晋妉,吴恒芳,陈相健,场笛..OX40/FOX40L逆向信号对小鼠主动脉内皮细胞MMP-9表达及机制的研究[J].南京医科大学学报（自然科学版）,2012,32(1):20-25,6.基金项目
国家自然科学基金项目(30772781,30770894) （30772781,30770894）
