南京医科大学学报（自然科学版）2012，Vol.32Issue(1)：109-114,6.
基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究
The relatedness in 60 drug-resistant Escherichia coil strains based on the analysis of the housekeeping genes and genes acquired by horizontal transfer
摘要
Abstract
Objective: To investigate the relatedness in 60 drug-resistant Escherichia coli (E.coli) strains. Methods; Drug-resistance related genes were analyzed by PCR,including 4 kinds of housekeeping genes and 45 kinds of horizontal transferred genes related to 3 kinds of antimicrobial agents:beta-lactams,aminoglycosides,quinolones,as well as 7 kinds of genetic markers of integrons, transposons, insertion sequences and conjugal plasmids. They were used for the sample clustering analysis. Results; Polymorphism were distinct in 60 E.coli strains. Most strains showed evolution. Only 3 strain groups which consisted of strain 6 and 53,strain 10 and 14,strain 7,8,26 and 40,were the same clones carried the same genes. Conclusion; Sample clustering analysis of drug-resistance related genes could distinguish the strains with the same antimicrobial resistance characteristics,which was significant in epi-demiologic research and provide convenience for tracing transmissible routes of drug-resistant strains.关键词
大肠埃希菌/菌株亲缘性Key words
Escherichia coli /relatedness分类
医药卫生引用本文复制引用
赵旺胜,王芳,翁幸鐾,糜祖煌,潘世扬..基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究[J].南京医科大学学报（自然科学版）,2012,32(1):109-114,6.基金项目
江苏省实验诊断学重点实验室重大项目(XK200731) （XK200731）
