南京医科大学学报（自然科学版）2012，Vol.32Issue(2)：172-176,5.
小檗碱对肾动脉粥样硬化发生、发展的干预作用
Effects of berberine on amelioration of renal artery damage caused by atherosclerosis
摘要
Abstract
Objective; To investigate the effect of high-cholesterol diet on renal artery atherosclerosis and the amelioration of berberine. Methods; Thirty SPF male Wistar rats were randomly divided into control group (n - 10),experimental group (n = 10) and berberine group (n = 10). Rats of control group were fed with normal diets while the other two groups were fed with high cholesterol diet starting from the second week for 3 months,after which the berberine group were administrated berberine (100 mg/kg) through intragastric administration daily,and the rats from experimental group were given normal saline for totally 6 weeks. Lidocaine was used for anesthesia and blood biochemical parameters such as cholesterol and creatinine were checked. Hematoxylin and Eosin (HE) staining and immunohistochemistry were used to investigate the effect of berberine on the AS lesion of renal artery. RT-PCR and Western blotting was used to study the expression of the IL-18 mRNA and protein. Results; Some of the blood biochemistry parameters of experimental group were significantly different from the other two groups (P < 0.01). The rats of experimental group have ■ generated a significant accumulation of the foam cells and migration of the smooth muscle cells compared with that of the control group,while rats treated with the berberine showed less accumulation of the foam cells and no significant migration of the smooth muscle cells. The relative expression of the IL-18 mRNA increased significantly after induction of renal artery AS compared with that of the control group and the berberine group (both P < 0.01). Conclusion; Berberine can suppress the expression of IL-18 and prevent the formation and development of the AS,consequently protect the renal artery,renal artery stenosis and the damage from renal artery stenosis.关键词
肾动脉粥样硬化/小檗碱/白介素-18Key words
renal artery atherosclerosis/berberine/interleukin-18分类
医药卫生引用本文复制引用
缪刚刚,朱一帆,陈文,张瑾,曹长春,陈鑫..小檗碱对肾动脉粥样硬化发生、发展的干预作用[J].南京医科大学学报（自然科学版）,2012,32(2):172-176,5.基金项目
中华医学会临床医学科研专项资金项目(09010290184) （09010290184）
