南京医科大学学报（自然科学版）2012，Vol.32Issue(2)：208-211,4.
庆大霉素-壳聚糖纳米粒治疗兔慢性骨髓炎的实验研究
Study of treatment of chronic osteomyelitis of rabbit with gentamicin loaded chitosan nanospheres
李亮亮 1王黎明 1金成哲 1徐燕 1唐成1
作者信息
- 1. 南京医科大学附属南京第一医院骨科,江苏南京210006
- 折叠
摘要
Abstract
Objective; To investigate the effect of gentamicin loaded chitosan nanospheres(GS/CS NPs) on the treatment of chronic osteomyelitis of rabbit. Methods: Forty-eight rabbits were used to establish the chronic osteomyelitis model,and observation of affected limb and X-ray were done 3 weeks later. Forty-four rabbits had symptoms and signs of chronic osteomyelitis and were divided into 4 groups. Group A has no treatment,group B was treated with chitosan nanospheres,group C was treated with gentamicin sulfate, and group D was treated with GS/CS NPs. The X-Ray,observation of the bone marrow cavity,bacterial culture and the HE staining were done after 3 weeks of treatment. Results-. After 3 weeks of treatment,the X-Ray of groups A~C showed apparent signs of chronic osteomyelitis,the formation of abscess were observed in bone marrow cavity,and the cultures of the Staphylococcus aureus were positive. The HE staining of groups A~C showed neutrophil accumulation, and the formation of dead bone were observed in groups A~B. The Norden and Smeltzer scores showed significant differences between group D and groups A~C (both P < 0.05). Conclusion; GS/ CS NPs has significant effects on the treatment of rabbit chronic osteomyelitis as local drug delivery system.关键词
庆大霉素/壳聚糖/纳米粒/兔/骨髓炎Key words
gentamicins/ chitosan/ nanospheres/ rabbit/ osteomyelitis分类
医药卫生引用本文复制引用
李亮亮,王黎明,金成哲,徐燕,唐成..庆大霉素-壳聚糖纳米粒治疗兔慢性骨髓炎的实验研究[J].南京医科大学学报（自然科学版）,2012,32(2):208-211,4.
