南京医科大学学报（自然科学版）2012，Vol.32Issue(2)：220-225,6.
实时荧光定量PCR法检测乳腺癌外周血循环肿瘤细胞的临床价值
The clinical value of detecting circulating tumor cells in peripheral blood of patients with breast cancer by real time QPCR
摘要
Abstract
Objective; To compare clinical value of different biomarkers (CK19,hMAM,SBEM) used to detect circulating tumor cells(CTCs) in peripheral blood of patients with breast cancer using real-time quantitative PCR (QPCR) platform. Methods; The expression of CK19,hMAM,SBEM in human breast cancer cell lines MCF-7 was detected by real time QPCR,peripheral blood samples of 20 healthy volunteers and 20 patients with breast benigh disease were detected to identify the base line expression of CK19, hMAM,SBEM. The expression of CK19,hMAM,SBEM in peripheral blood from 94 patients with operable breast cancer was detected also. Results; CK19,hMAM and SBEM were all detected in MCF-7,and the Ct value were 13.87,34.47,and 20.14, respectively ;a-mong the 40 control persons,one sample expressed CK19,one sample expressed hMAM.and no sample expressed SBEM;among the 94 patients with operable breast cancer,46(49%) samples expressed CK19,32(34%) samples expressed hMAM,and 12(13%) samples expressed SBEM,53(56%) samples were positive by combined detection using the three biomarkers. Conclusion; It is higher sensitivity to detect circulating tumor cells in peripheral blood of breast cancer combined detection using multimarkers (CK19, hMAM,SBEM) based on a real time QPCR platform,yet specificity was not depressed.关键词
乳腺癌/循环肿瘤细胞/细胞角蛋白19/乳珠蛋白/乳腺小黏蛋白Key words
breast cancer/ circulating tumor cell /CK19/hMAM/ SBEM分类
医药卫生引用本文复制引用
崇梅红,王水,查小明,刘晓安,凌立君,肇毅..实时荧光定量PCR法检测乳腺癌外周血循环肿瘤细胞的临床价值[J].南京医科大学学报（自然科学版）,2012,32(2):220-225,6.基金项目
国家自然科学基金(81071753) （81071753）
江苏省六大人才高峰项目(2010-ws-079) （2010-ws-079）
