南京医科大学学报（自然科学版）2012，Vol.32Issue(2)：236-240,5.
Notch1和HER2在胃癌中的表达及临床意义
Expression and clinical significance of Notch1 and HER2 in gastric carcinoma
刘标 1孙利兵 1陈伟群 1周俊东 2吴锦昌2
作者信息
- 1. 南京医科大学附属苏州医院病理科
- 2. 肿瘤中心,江苏苏州215001
- 折叠
摘要
Abstract
Objective: To study the expression and clinical significance of Notchl and human epidermal growth factor receptor 2 (HER2) in gastric carcinoma. Methods; The expression of Notchl was detected by immunohistochemistry,and assessment of HER2 protein overexpression and gene amplification were done by immunohistochemistry and fluorescence in situ hybirdizaton (FISH) in tissue microarrays containing 317 gastric carcinomas and relatively normal gastric mucosa tissue. Results; Overexpression of HER2 protein was observed in 64 cases(20.2%) and HER2 gene amplification was detected in 58 cases(18.3%) of gastric carcinoma. The concordance rate between the immunohistochemistry and FISH was 89.1%. HER2 protein overexpression and gene amplification were significantly associated with tumor location and the Lauren's classification (P < 0.01),but not with differentiation grade and clinical stage (P > 0.05). The expression of Notchl protein was observed in 197 cases (62.1%) of gastric carcinoma,and was significantly associated with differentiation grade and clinical stage (P < 0.05). The expression of Notchl was negatively correlated to the protein overexpression and gene amplification of HER2 (r = -0.191 ,P < 0.01 ;r = -0.169,P < 0.01 .respectively). Conclusion; The significantly dys-regulated expression of Notchl,which could promote growth and invasion of gastric carcinoma cells and correlated to the higher clinical TNM stage,was observed in gastric carcinoma. The expression of Notchl negatively correlated to the protein overexpression and gene amplification of HER2 suggests that Notchl may act as a novel therapeutic target molecule for gastric carcinoma.关键词
胃癌/Notch1/人类表皮生长因子受体2/免疫组化/FISHKey words
gastric carcinoma/Notchl/human epidermal growth factor receptor 2/immunohistochemistry/FISH分类
医药卫生引用本文复制引用
刘标,孙利兵,陈伟群,周俊东,吴锦昌..Notch1和HER2在胃癌中的表达及临床意义[J].南京医科大学学报（自然科学版）,2012,32(2):236-240,5.
