内科理论与实践2012，Vol.7Issue(1)：38-41,4.
脂联素球状结构域对3T3-L1脂肪细胞磷酸戊糖途径关键酶表达的影响
Effect of globular domain of adiponectin on pentose phosphate pathway key enzyme of 3T3 adipocyte
摘要
Abstract
Objective To investigate the effect of globular domain of adiponectin (gAd) on pentose phosphate pathway key enzyme of 3T3-L1 adipocytes. Methods Matured 3T3-L1 adipocytes were intervened with gAd, and realtime PCR (RT-PCR) was used to determine the transcription level of glucose-6-phosphatase (G6PD), the key enzyme in pentose phosphate pathway; the changes of transcription level of G6PD were expressed in changes of mRNA levels. All the data were analyzed statistically. Results G6PD transcription level was not different between gAd group and control group. Conclusions gAd promotes the intake of glucose by 3T3-L1 cells, and the catabolism of glucose taken probably was not via the pentose phosphate pathway.Objective To investigate the effect of globular domain of adiponectin (gAd) on pentose phosphate pathway key enzyme of 3T3-L1 adipocytes. Methods Matured 3T3-L1 adipocytes were intervened with gAd, and realtime PCR (RT-PCR) was used to determine the transcription level of glucose-6-phosphatase (G6PD), the key enzyme in pentose phosphate pathway; the changes of transcription level of G6PD were expressed in changes of mRNA levels. All the data were analyzed statistically. Results G6PD transcription level was not different between gAd group and control group. Conclusions gAd promotes the intake of glucose by 3T3-L1 cells, and the catabolism of glucose taken probably was not via the pentose phosphate pathway.关键词
脂联素球状结构域/3T3-L1脂肪细胞/关键酶/磷酸戊糖途径Key words
Globular domain of adiponectin/ 3T3-L1 adipocytes/ Key enzyme/ Pentose phosphate pathway分类
医药卫生引用本文复制引用
王彦,廉如,陈思思,高婷婷,吴彬,陈显久..脂联素球状结构域对3T3-L1脂肪细胞磷酸戊糖途径关键酶表达的影响[J].内科理论与实践,2012,7(1):38-41,4.基金项目
山西省科技攻关项目(项目编号:20090321104) （项目编号:20090321104）
