畜牧与饲料科学Issue(9)：30-34,5.
果香味剂对奶牛乳腺上皮细胞培养液中乳成分含量及风味影响的研究
Study on Effects of Mixed Fruit Flavor Meal on Milk Composition Content and Flavor inDairy Cow Mammary Epithelial Cell Culture Liquid
摘要
Abstract
The effects of mixed fruit flavor meal on milk composition content and flavor in dairy cow mammary epithelium cell culture liquid were studied.The dairy cow mammary epithelial cells were home-made in this trial.There were 5 groups including 1 control group and 4 treatment groups.The mixed fruit flavor meal dosage in culture liguid of mammary epithelial cell was 0,5,10,15 and 20 μg/mL,respectively.The result indicated：① The milk total protein,fat and lactose contents were highest for 15 μg/mL dosage group,the total protein and lactose contents were not affected significantly（P 0.05）,but fat content was affected significantly by adding the mixed fruit flavor meal（P0.05）.The milk composition content was gradually declining with the mixed fruit flavor meal dosage,which indicated the fruit flavor improving the secretion level of milk protein,fat and lactose.②The flavor compositions of mammary epithelial cell culture liquid were analyzed by the purge and trap-gas chromatograph and mass spectrum（PT/GC-MS）.Except for the inherent flavor of mammary gland culture liquid,the other flavor composition were also found,such as Acetic acid,2-methylpropyl ester;1-Butanol,3-methyl-,acetate;Butanoic acid,2-methylpropyl este;Butanoic acid,ethyl ester;1-Butanol,3-methyl-,propanoate;Propanoic acid,pentyl ester;Butanoic acid,3-methylbutyl ester;Butanoic acid,pentyl ester,and so on.And these compounds were from the mixed fruit flavor meal,the content of flavor compounds was highest for 15 μg/mL dosage group,then was gradually declining.In conclusion：the mixed fruit flavor meal could significantly affect milk composition and the flavor of mammary epithelial cell culture liquid.关键词
果香味剂/乳腺上皮细胞/乳成分/培养液风味Key words
mixed fruit flavor meal/mammary epithelial cell/milk composition/flavor分类
农业科技引用本文复制引用
马燕芬,高民,卢德勋..果香味剂对奶牛乳腺上皮细胞培养液中乳成分含量及风味影响的研究[J].畜牧与饲料科学,2011,(9):30-34,5.基金项目
内蒙古自治区科技厅重大专项项目 （）
农业部公益性行业专项项目 （）
现代农业产业技术体系 （）
