畜牧与饲料科学Issue(9)：49-53,58,6.
饲粮不同NFC/NDF对奶山羊瘤胃溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌含量变化的影响
Effect of Different Dietary NFC/NDF on Populations of B. fibrisolvens,S. bovis and M. elsdenii in Rumen of Dairy Goats
摘要
Abstract
The objective of this experiment was to investigate the effect of increasing dietary NFC/NDF on populations of B. fibrisolvens,S. bovis and M. elsdenii in the rumen of dairy goats under the condition of induced subacute rumen acidosis （SARA）.Six lactating Guanzhong dairy goats fitted with permanent rumen fistulae were used in a self-contrast experiment design.This experiment consisted of four experimental periods,with ten days in each period.Goats were fed 4 diets with different NFC/NDF （1.02,1.24,1.63 and 2.58,respectively）.The induction of SARA was conducted by gradually increasing dietary NFC/NDF.Realtime PCR was used to detect the populations of B. fibrisolvens,S. bovis and M.elsdenii.The results showed as follows：① The number of B. fibrisolvens and M. elsdenii was both increased with the increasing of dietary NFC/NDF,and when NFC/NDF reached the level of 2.58,the number of M. elsdenii was significantly increased （P0.01）,whereas the number of B. fibrisolvens was significantly decreased （P0.01）.② The number of S. bovis was significantly decreased with the increasing of dietary NFC/NDF （P0.05）,however,during the occurrence of SARA,the number of S. bovis returned to the same level of experimental period Ⅰ.The results indicated that there was no difference in the number of S. bovis at the induced stage of SARA,however,the number of B. fibrisolvens was markedly decreased,whereas the number of M. elsdenii, which is a kind of aciduric bacteria, was significantly increased.关键词
饲粮NFC/NDF比/亚急性瘤胃酸中毒/实时定量PCR/瘤胃细菌Key words
dietary NFC/NDF/SARA/realtime PCR/rumen bacteria分类
农业科技引用本文复制引用
赵培厅,刘大程,高民,胡红莲,韩昊奇,周向丽,邓维康,王鹏飞..饲粮不同NFC/NDF对奶山羊瘤胃溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌含量变化的影响[J].畜牧与饲料科学,2011,(9):49-53,58,6.基金项目
国家自然科学基金项目 （）
现代农业产业技术体系建设专项 （）
