畜牧与饲料科学Issue(9)：65-67,71,4.
黑白花奶牛乳腺上皮细胞冻存方法的研究
Study on the Cryopreservation Method of Holstein Cow Mammary Epithelial Cells
摘要
Abstract
The effects of different cryopreservation methods of Holstein cow mammary epithelial cells on its viability were studied. Primary bovine mammary epithelial cells were passing five generations and adjusting the cell density to 1×106 cells/mL,and then being cryopreserved by 6 different methods.The viability of cells including mortality,adhering dish rate,apoptosis rate was measured after being recovered.The results showed that the death rate of cell being cryopreserved by treatments 2,3,5 was significantly different from treatment 1（P0.01）.Whereas no significant difference was found among treatments 1,4 and 6（P 0.05）.There was significantly different for death rate of cryopreserved cell between treatment 2 and 6（P0.01）.Adhering rate of cryopreserved cell from treatment 4 was significantly higher than treatment 2,5 and 6（P0.01）.Cell growth state of treatment 1 and 4 were better than others. The results indicated that the treatment 4 was the best cryopreservation method in this study.关键词
冻存/贴壁率/凋亡率/乳腺上皮细胞Key words
cryopreservation/adhering rate/apoptosis rate/mammary epithelial cell分类
农业科技引用本文复制引用
王秀美,考桂兰,侯先志,高爱武,杨苗,杨银芬..黑白花奶牛乳腺上皮细胞冻存方法的研究[J].畜牧与饲料科学,2011,(9):65-67,71,4.基金项目
农业部“奶牛产业技术体系”项目子课题 （）
