农业环境科学学报2011，Vol.30Issue(12)：2434-2439,6.
酸铝胁迫对栝楼根系生长及铝积累的影响
Effects of Acid Aluminum Stress on Root Growth and Aluminum Accumulation of Trichosathes kirilowii Maxim
摘要
Abstract
Adverse effect on crop growth is caused by south red soil with high Al concentration. The aim of this work is to investigate the effects of acid aluminum stress on root growth and aluminum accumulation of Trichosdthes kirilowii Maxim by water planting. Seedlings were treated with different concentrations, 0, 100, 300, 500, 1 000 μmol·L-1 of aluminium chloride, at pH 4.5 for 7 d. Following treatment the parameters investigated in root tissue were root vigor, plasma membrane permeability, activities of antioxidant enzymes named peroxidase (POD), catalase(CAT), ascorbate peroxidase(APX), guaiacol peroxidation(GPX), superoxide dismutase(SOD) and their isozyme elec-trophoresis, hydrogen peroxide content, relative aluminum content. With the increasing of Al3+ concentration, the activities of POD, CAT and APX increased but the activities GPX and SOD decreased; most of the antioxidant enzymes had several electrophoretic bands; plasma membrane permeability had no significant difference; root vigor increased remarkably. Determining relative content of aluminum had the same result by comparing with that of morin staining more aluminum accumulated in the cells of root hair than that in closely arranging cells. Antioxidant enzymes system and aluminum accumulation mechanism play important role in resisting Al toxicity for the growth of T. Kirilowii.关键词
铝胁迫/栝楼/根系生长/根尖铝含量Key words
aluminum stress/ Trichosathes kirilowii Maxim/ root growth/ relative aluminum content分类
农业科技引用本文复制引用
周媛,章艺,吴玉环,徐根娣,高培培,姚国浩,刘鹏..酸铝胁迫对栝楼根系生长及铝积累的影响[J].农业环境科学学报,2011,30(12):2434-2439,6.基金项目
浙江省自然科学基金(Y5100390) （Y5100390）
浙江省公益技术研究农业项目(2011C22053) （2011C22053）
