农业机械学报2011，Vol.42Issue(5)：156-159,159,5.
板栗壳中原花青素大孔吸附树脂分离纯化工艺优化术
Separation and Purification Procyanidins from Chestnut-shells by Macroporous Resin
摘要
Abstract
With the aim to separate and purify procyanidins from chestnut-shells ( CSPCs) by macroporous resin, twelve macroporous resins were tested for their static adsorption/desorption performance towards CSPCs. Based on the static adsorption experiments, the suitable macroporous resin was screened to carry out dynamic adsorption experiment, the content and the relative molecular weight of CSPCs were analyzed. The results showed that AB - 8 was the optimal macroporous resin for separating and purifying CSPCs, the optimum separating condition was as follows; the mass concentration and the sampling rate were 1. 864 mg/mL and 2 mL/min, respectively. The total recovery of CSPCs reached 94. 2% eluted with 50% ethanol of 4 BV at a flow velocity of 1 mL/min, and the content of the total CSPCs in the obtained fine product was 89. 8% . Analysis of CSPCs by ESI - MS demonstrated the molecular weight ranged from 289. 4 to 1 154. 9 ( M + H) within 4 of average polymeric degree.关键词
原花青素/板栗壳/大孔吸附树脂/分离纯化Key words
Procyanidins/ Chestnut-shells/ Macroporous resin/ Separation and purification分类
轻工纺织引用本文复制引用
张海晖,段玉清,李金凤,马海乐,邵婷婷,徐菲菲..板栗壳中原花青素大孔吸附树脂分离纯化工艺优化术[J].农业机械学报,2011,42(5):156-159,159,5.基金项目
国家自然科学基金资助项目(309700309)、国家博士后基金资助项目(20100471379)、江苏省高校自然基金资助项目(05KJB550011)、江苏大学人才基金资助项目(05JDG013、128300289)和江苏高校优势学科建设工程资助项目 （309700309）
