农业机械学报2011，Vol.42Issue(6)：147-151,5.
野生酸枣果硒多糖纯化与光谱分析
Purification and Corresponding Spectroscopic Analysis of Selenium Polysaccharide in Sour Jujube Fruits
摘要
Abstract
The crude polysaccharide was extracted from sour jujube fruits using hot water extraction followed by ethanol precipitation. The content of crude polysaccharide was ( 92. 6 ± 4. 72 ) mg/g by phenol-sulfuric acid assay, and selenium content of the polysaccharide was (17.8 ±2.05) βg/kg by fluorescence spectrophotometry method. After further purified by DEAE - 52 and Sephadex G - 75 column chromatography, a white and flocculent polysaccharide was obtained. After hydrolysis with 2M trifluoroacetic acid, TLC plates was detected and high performance anion exchange chromatography with pulsed amperometric detection ( HPAEC-PAD ) was further analyzed. A comparative study of the two methods showed that the polysaccharide was composed of rhamnose, xylose, arabinose, glucose and galactose with a ratio of 3. 21: 8. 36= 5. 63: 0. 96= 1. 82. The selenium polysaccharide exhibited typical absorption of polysaccharide characterized by Infrared spectroscopy ( IR) spectroscopy; 1 H NMR and C NMR analysis indicated that the structure of selenium polysaccharide was pyranoid glucan with β-glycosidic bond.关键词
酸枣/硒/多糖/高效阴离子交换色谱/红外光谱/核磁共振波谱Key words
Sour jujube/Selenium/Polysaccharide/High performance anion exchange chromatography/Infrared spectroscopy/Nuclear magnetic resonance分类
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孙延芳,梁宗锁,单长卷,ViernsteinH,UngerF..野生酸枣果硒多糖纯化与光谱分析[J].农业机械学报,2011,42(6):147-151,5.基金项目
中国科学院知识创新项目(KZCX2-XB2-05-01)和"十一五"国家科技支撑计划资助项目(2008BAD98B08) （KZCX2-XB2-05-01）
