农业生物技术学报2011，Vol.19Issue(5)：785-792,8.DOI:10.3969/j.issn.1674-7968.2011.05.001
利用三重测交群体剖析玉米苗期性状杂种优势的遗传学基础
Heterosis for Seedling Traits in Maize (Zea mays L.) revealed by Quantitative Trait Loci Analysis of the Triple Testcross Design
摘要
Abstract
To investigate the genetic basis of heterosis in maize seedling traits, highly heterotic maize (Zea mays L.) hybrid Yuyu22 and RILs (recombinant inbred lines) were used as basic population to construct TTC (triple testcross) population, which included 312 testcross progenies by using TTC genetic mating design. Thirty QTLs were detected for the longest root length (LRL), shoot height (SH), primary root number (RN), root dry matter weight (RDW) and shoot dry matter weight (LDW) by using composite interval mapping. And some QTLs for different seedling traits were found to be located on the same chromosome regions, and a total of 4 regions were detected, which distributed on chromosome 2, 3 and 7, respectively. Further analysis indicated that 22 QTLs were detected by using Zr and Zq data, which were classified as overdominant(l 1), additive(5), partially dominant(5) and dominant (1). Furthermore, 8 genome regions of QTLx genetic background interactions and 16 markers pairs with epistatic effects were detected. Collectively, we propose that overdominance and epistasis are the main genetic basis of maize seedling traits and their heterosis.关键词
玉米/苗期性状/三重测交/杂种优势/QTL定位Key words
Maize/ Seedling traits/ Triple testcross/ Heterosis/ QTL引用本文复制引用
宋方威,彭惠茹,张义荣,杨小红,刘婷,孙其信,倪中福..利用三重测交群体剖析玉米苗期性状杂种优势的遗传学基础[J].农业生物技术学报,2011,19(5):785-792,8.基金项目
本研究由国家重点基础研究发展计划(973)项目(No.2007CB 109000)、国家杰出青年科学基金(No.30925023)和国家自然科学基金(No.30671297,No.30771342)共同资助 （973）
