农业生物技术学报2011，Vol.19Issue(5)：816-822,7.DOI:10.3969/j.issn.1674-7968.2011.05.005
花生二氢黄酮醇还原酶基因(DFR)的克隆及表达分析
Cloning of Dihydroflavonol 4-reductase Gene (DFR) from Peanut (Arachis hypogaea L.) and Its Expression Analysis
摘要
Abstract
Dihydroflavonol 4-reductase (DFR) is a key enzyme converting dihydroflavonols to leucoanthocyanidins in the biosynthesis of anthocyanins. EST sequencing was carried out using a cDNA library of peanut(A rachls hypogaea L.) immature seed and full length cDNA of peanut DFR was cloned. Sequence alignment showed that DFR was highly conserved among different plant species. Peanut cDNA microarray and semi-quantitative RT-PCR were used to analysis the expression of DFR in different tissues. Results indicated that the expression level was highest in gynophores and followed by flowers, while the expression in roots and leavies was low. DFR gene expression was also analysed in seeds from different cultivars, which suggested that it expressed higher in cultivars with dark-color seed coat, the expression was the highest in ZH9 seeds with black seed coat. We also analysed the expression level of DFR in a peanut mutation with purple leaves and stems, which demonstrated that DFR was highly expressed in the purple tissues. These results demonstrate that the expression level of DFR gene is positively correlation to the accumulation of anthocyanins, which indicates that DFR catalytic reaction is the key step in the biosynthesis of anthocyanins.关键词
花生/cDNA文库/花青素/DFR/基因表达Key words
Arachis hypogaea L./ cDNA library/ Anthocyanin/ DFR/ Gene expression引用本文复制引用
郭凤丹,夏晗,袁美,王兴军..花生二氢黄酮醇还原酶基因(DFR)的克隆及表达分析[J].农业生物技术学报,2011,19(5):816-822,7.基金项目
本研究由国家自然科学基金(No.30871324,No.31000720)、山东省优秀中青年科学家奖励基金(No.2006BS06008)、山东省自然科学基金(No.Y2008D44)和山东省"泰山学者"专项基金共同资助 （No.30871324,No.31000720）
