农业生物技术学报2011，Vol.19Issue(5)：881-886,6.DOI:10.3969/j.issn.1674-7968.2011.05.014
血管紧张素转化酶2(ACE2)对大鼠肾氧化应激损伤的保护作用及其机制
Protective Effect and Mechanism of Angiotensin Converting Enzyme 2 (ACE2) on Renal Oxidative Stress Injury in Rats
摘要
Abstract
The objective of this study was to investigate the protective effect and mechanism of angiotensin converting enzyme 2(ACE2) on renal oxidative stress injury induced by high glucose. The iats(Rattus norregicus) model of renal oxidative stress injury was established by intraperitoneal injection of streptozotocin(STZ) solution, and treated by subcutaneous injection of insulin once a day(l mL (3.7xl0-5 mol/L)). After 30 days, all the rats were decapitated and the serum and kidney tissue were collected. The contents of advanced glycation end products (AGEs), malondialdehyde(MDA), superoxide dismutase(SOD), angiotensin II (Angll), angiotensinl-7(Angl-7) in serum and the mRNA levels of A CE2 and Mas receptor in kidneys were measured. Compared with control group, the contents of AGEs, MDA and Angll in serum of high glucose rats were significantly increased(P<0.01), and the levels of SOD and Angl-7 in serum were significantly decreased (P<0.01). The mRNA expression of A CE2 in kidneys was obviously reduced (P<0.01), while the mRNA expression of Mas was obviously raised (P<0.01). After insulin treatment, the contents of AGEs and MDA in serum were obviously reduced (P<0.01); the activity of SOD in serum was significantly raised (P<0.01); the level of Angll in serum was significantly decreased (P<0.05) while the concentration of Angl-7 in serum was significantly increased (P<0.05). The mRNA expression levels of A CE2and Mas receptor in kidneys were significantly increased (P<0.01). Exparemental results suggest that ACE2 plays an important role on the renal oxidative stress injury induced by high glucose and the mechanism may be that the activation of the ACE2-Angl-7-Mas axis reduces the effect of Ang Ⅱ.关键词
血管紧张素转化酶2(ACE2)/大鼠/肾脏/氧化应激/保护作用Key words
Angiotensin converting enzyme 2 (ACE2)/ Rat/ Kidney/ Oxidative stress/ Protective effect引用本文复制引用
王艳霞,张伟,韩东宁,张源淑..血管紧张素转化酶2(ACE2)对大鼠肾氧化应激损伤的保护作用及其机制[J].农业生物技术学报,2011,19(5):881-886,6.基金项目
本研究由国家白然科学基金(No.30871838-A200850)资助 （No.30871838-A200850）
