农业生物技术学报2011，Vol.19Issue(6)：981-987,7.DOI:10.3969/j.issn.1674-7968.2011.06.001
牙鲆微卫星标记遗传连锁图谱的构建
Construction of a Microsatellite-based Genetic Linkage Map in Japanese flounder (Paralic hthys olivace us )
摘要
Abstract
For carrying out molecular marker or gene assistant selective breeding in Japanese flounder cultivation, and increasing commercial production of Japanese flounder, we used simple sequence repeat (SSR) markers to construct the primary linkage map in a family of Japanese flounder in China. One thousand novel microsatellite markers for Japanese flounder (Paralichthys olivaceus) were selected randomly from a microsatellite-enriched library constructed by genome sequencing for designing primers. The genetic linkage map with SSR markers in Japanese flounder was constructed using 2009 established No. 10 families as mapping population with the JoinMap4.0 software. Both of the male and female maps were formed by 24 linkage groups. The female map has 212 markers with a total length of 1 320.4 cM, 77.7% coverage. The male map has 198 markers with a total length of 1 361 cM, 76.1% coverage. The length of each linkage group varied from 9.3 to 11.6.1cM and the maker number in these linkage groups was from 3 to 21. However, The distribution of SSR markers in each linkage group was not even and high density region of markers existed on linkage group 1, 3 and 8. The improved genetic linkage maps are very useful for QTL analyses and marker assisted selection (MAS) breeding programs in Japanese flounder.关键词
牙鲆/遗传连锁图谱/微卫星/分子标记辅助育种Key words
Paralichthys olivaceus/ Genetic linkage map/ Simple sequence repeat(SSR)/ Marker assisted selection引用本文复制引用
宋文涛,梁卓,田永胜,陈松林,张潇峮,廖小林,邵长伟,王磊,庞仁谊,牛余泽,赵永伟,李文龙..牙鲆微卫星标记遗传连锁图谱的构建[J].农业生物技术学报,2011,19(6):981-987,7.基金项目
本研究由公益性行业(农业)科研专项(No.200903046)、国家基础研究发展规划(973)项目(No.2010CB126303)、国家自然科学基金项目(No.30871918)和山东省泰山学者工程专项共同资助 （农业）
