农业生物技术学报2011，Vol.19Issue(6)：1019-1026,8.DOI:10.3969/j.issn.1674-7968.2011.06.006
丁内酯-Ⅰ影响猪卵母细胞的体外成熟和发育能力
Effects of Butyrolactone Ⅰ on Maturation and Development Competence of Porcine Oocytes In vitro
摘要
Abstract
There is not synchronized between nuclear maturation and cytoplasmic maturation of in vitro maturated oocytes. In order to solve this problem, we use butyrolactone I (BL-1), cdc2 kinase inhibitor, to prevent porcine oocyte nuclear maturation in vitro. Porcine oocytes were cultured for 44 h in the medium containing different concentrations of BL- I (0, 10, 20, 40 and 80 mol/L, respectively). With the increase of BL- I concentration, the inhibition efficiency on maturation of porcine oocytes was increased. When treated with BL- I in 20, 40 and 80 mol/L, respectively, the oocytes reached 25%, 47% and 67%, respectively, at germinal vesicle (GV) stage, and 38%, 9% and 0% at Mil stage, which were both significant difference with control group. In addition, After removed BL-1 and incubated for additional 44 h in ooycte maturation media, these oocytes at MII stage were significant increased and reached 51%, 47% and 5%, respective, which showed the BL- I inhibition to maturation of oocytes is reversible. Additionally, oocytes incubated with 20 (Jimol/L BL-1 for 20 and 24 h without BL- I (20 h++24 h "treatment group), showed low percentage at M II stage compared with the control group (culture for 44 h without BL- I ) (P < 0.05, 65.6% vs 70.1%). When the matured oocytes from 20 h++24 h" treatment group were parthenogenetic activated, the cleavage rate, blastocyst rate and blastocyst total cell number had increased, but no significant difference compared with the control group. When the matured oocytes were used for nuclear transfer, the cleavage rate, blastocyst rate and blastocyst total cell number were better than that of the control group, but only significant difference in cleavage rate (P <0.05, 69% vs 62%). These results indicate that BL- I can significantly inhibit the porcine oocytes maturation, and that the inhibition can be reversed by removing BL- I . These results also show that matured oocytes treated with the proper concentration of BL- I can slightly improved the development ability of parthenogenetic and nuclear transfer embryos.关键词
猪/卵母细胞/丁内酯-Ⅰ(BL-Ⅰ)/体外成熟/核移植胚胎Key words
Porcine/ Oocyte/ Butyrolactone I / In vitro maturation/ NT embryo引用本文复制引用
鲍建昌,高星,胡军和,马晓玲,王华岩..丁内酯-Ⅰ影响猪卵母细胞的体外成熟和发育能力[J].农业生物技术学报,2011,19(6):1019-1026,8.基金项目
本研究由国家高技术研究发展计划(863)(No.2008AA10005)和国家重点基础研究发展规划(973)(No.2009CB941002)共同资助 （863）
