农业生物技术学报2011，Vol.19Issue(6)：1034-1041,8.DOI:10.3969/j.issn.1674-7968.2011.06.008
表皮生长因子受体基因(EGFR)多态性及其与山羊产羔数的相关分析
Polymorphism of Epidermal Growth Factor Receptor Gene (EGFR) and Its Relationship with Litter Size in Goats
摘要
Abstract
The epidermal growth factor receptor have been identified as a key players in female reproductive functions, the objectives of the present study were to identify the polyorphism of epidermal growth factor receptor gene (EGFR) and find the association between reproductive traits and the gene in goats. According to the Bos taurus EGFR gene sequence provided by The National Center for Biotechnology Information, we designed fourteen pairs of primers to detect single nucleotide polymorphisms of EGFR gene in Matou, Boer, Gulin Ma, Chuandong White, Dazu Black, Guizhou White, Jintang Black, Banjiao, Chengdu Ma, Nanjiang Brown of goats (Capra hircus) by PCR-single strand conformation polymorphism (SSCP). The result showed that the DNA fragments amplified by primer P3 and primer P13 had polymorphisms among primers P1~P14. For the fragments amplified by primer P3, three genotypes (AA, BB and AB) were detected in different goat breeds. The sequencing results revealed that the genotype BB had one point mutation (115837C→T) compared to AA genotype. The litter size with genotype AA and genotype AB had no significant difference in three breeds of goats (P〉0.05), and goats with BB genotype rarely existed. For primer PI 3, three genotypes (GG, GH and HH) were detected in different goat breeds. The sequencing results revealed that the genotype HH had one point mutation (I73015G→A) compared to GG genotype. The goats with genotype GH had significant higher litter size than those with genotype GG in Guizhou White and Chuandong White goat (P<0.05), but Gulin Ma didn't have polymorphisms of primer PI3, and goats with HH genotype rarely existed. The results initially indicate that the EGFR gene may be either a major gene that influences the prolificacy in goats or a molecular marker linked with such a gene closely.关键词
山羊/繁殖力/表皮生长因子受体基因(EGFR)/PCR-SSCPKey words
goat/ prolificacy/ F.GFR gene/ PCR-SSCP引用本文复制引用
宋文静,王凭青,张宝云,储明星,孔祥军,谭颖,樊奇,刘重旭..表皮生长因子受体基因(EGFR)多态性及其与山羊产羔数的相关分析[J].农业生物技术学报,2011,19(6):1034-1041,8.基金项目
本研究由国家现代农业产业技术体系建设专项(No.CARS-39)、重庆市自然科学基金重点项目(CSTC,2009BA1066)和中央高校基本科研业务费(CDJXS11230004)共同资助 （No.CARS-39）
