中国海洋大学学报（自然科学版）2011，Vol.41Issue(12)：81-85,5.
一株海绵共附生真菌Pleosporaceaesp.中甾体类化合物及其生物活性
The Steroids and Their Bioactivities of a Sponge-Derived Fungus Pleosporaceae sp.
摘要
Abstract
Under the guidance of bioassay, five steroids were isolated from the ethyl acetate extract of a sponge-derived fungus Pleosporaceae sp. By column chromatography on silica gel and Sephadex LH-20 and preparative-HPLC, Their structures were elucidated as (22E, 24jR)-5α,8α-epidioxy-ergosta-6, 22-dien-3β-ol (1), (22E,24R)-5α,8α-epidioxy-ergosta -6,9,22-trien-3β-ol (2), (22E)-ergosta-5,7,22-trien-3β-ol (3), ergosta-6α-hydroxy-4,22-dien-3-one (4) and (22E)-5crcholesta-7,22-diene-3β,5α,6β-triol (5), respectively, on the basis of their spectroscopic data including NMR and MS, combining with the data of literatures. All of the compounds were isolated from the genus of Pleosporaceae for the first time. Compounds 1 and 5 exhibited strong lethality to brine shrimp Artemia salina. And compound 1 showed potent antifouling activity against the larval settlement of barnacle Balanus amphitrite with the EC50 of 0. 85 μg/mL.关键词
海绵共附生真菌/次级代谢产物/甾体/生物活性Key words
sponge derived fungus/ secondary metabolites/ steroids/ bioactivity分类
医药卫生引用本文复制引用
赵琨,孙雪萍,韩磊,郑彩娟,陈敏,邵长伦,佘志刚,王长云..一株海绵共附生真菌Pleosporaceaesp.中甾体类化合物及其生物活性[J].中国海洋大学学报（自然科学版）,2011,41(12):81-85,5.基金项目
国家自然科学基金项目(40776073 （）
30901879) （）
国家科技基础性工作专项(2007FY210500) （2007FY210500）
国家教育部高等学校博士学科点专项(20090132110002)资助 （20090132110002）
