热带作物学报2011，Vol.32Issue(8)：1475-1478,4.DOI:10.3969/j.issn.1000-2561.2011.08.018
测定生姜蛋白酶活性的新方法
A New Method for Detecting Ginger Protease Activity
摘要
Abstract
In this paper, a new method for detecting ginger protease activity was proposed by using inactive ginger protease as a reference. Coomassie brilliant blue staining method was employed to detect the activity of ginger protease. The influence of some factors, such as the pH, temperature, metallic ions (Na+, Mg2+, Cu2+, Ca2+) and EDTA on the activity of the protease was discussed. The results indicate that taking the inactive ginger protease as a reference could detect the activity of ginger protease laconically, which exhibited the advantages of simplicity and avoiding the harmfulness of corrosive substance as compared to other detecting method.关键词
生姜蛋白酶/加热使酶失活/参照/酶活测定/考马斯亮蓝比色法Key words
Ginger protease/ Inactivate the enzyme by heating/ Reference/ Determination of enzyme activity/Coomassie brilliant blue colorimetric method.分类
生物科学引用本文复制引用
于洁,肖超妮,王世祥,郑晓晖..测定生姜蛋白酶活性的新方法[J].热带作物学报,2011,32(8):1475-1478,4.基金项目
陕西省教育厅自然科学项目(09JK788) （09JK788）
陕西省中医管理局09年项目(zy58). （zy58）
