热带作物学报2011，Vol.32Issue(8)：1488-1493,6.DOI:10.3969/j.issn.1000-2561.2011.08.021
橡胶树铜转运蛋白基因的克隆及在健康树与死皮树中的差异表达
Cloning of the Copper Transporter Gene from Hevea brasiliensis and Analysis of the Differential Expression Profile between Healthy Trees and Tapping Panel Dryness Trees
摘要
Abstract
High-affinity copper transporter (COPT) is the key member of CTR/COPT family, which plays an important role in mediating copper homeostasis in organisms. Using the primers designed from the known EST from a suppression subtractive hybridization -Cdna library, the full -length Cdna sequence of HbCOPT5 was cloned from Hevea brasiliensis latex by 3-RACE technique. The opening reading frame(ORF)of HbCOPT5 was 420 bp in length, encoding 139 amino acids. HbCOPT5 was predicted to contain three transmembrane domains (TMDs) with molecular weight 15.67 ku and theoretical Pi 5.26. Phylogenetic reconstruction revealed that HbCOPT5 was more closely related to the COPTs from Ricinus communis and Populus trichocarpa, and most closely related to the Arabidopsis COPT5 among the AtCOPTl-AtCOPT6 transporter family. In addition, HbCOPTS exhibited 2.8 times higher transcriptional expression level in healthy trees than in tapping panel dryness trees.关键词
巴西橡胶树/死皮/铜转运蛋白/克隆/表达分析Key words
Hevea brasiliensis/ Tapping panel dryness/ Copper transporter/ Cloning/ Expression analysis分类
农业科技引用本文复制引用
喻修道,王真辉,袁坤,邹智,杨礼富..橡胶树铜转运蛋白基因的克隆及在健康树与死皮树中的差异表达[J].热带作物学报,2011,32(8):1488-1493,6.基金项目
中国热带农业科学院橡胶研究所基本科研业务费专项资金资助项目(No.YWFZX2010-9). （No.YWFZX2010-9）
