热带作物学报2011，Vol.32Issue(8)：1494-1498,5.DOI:10.3969/j.issn.1000-2561.2011.08.022
橡胶树体细胞胚胎发生相关类受体蛋白激酶基因[HbSERK1)的克隆及表达分析
Cloning and Expression of HbSERK1 from Hevea brasiliensis
摘要
Abstract
The full-length cDNA encoding somatic embryogenesis receptor-like kinase, designated HbSERKl, was isolated from rubber tree. HbSERKl was a 2 159 bp long containing a 1 482 bp open reading frame encoding a putative protein of 493 amino acids. The deduced amino acid sequences of HbSERKl shared 91%, 90%, 88%, 88%, 87% and 86% identity with the somatic embryogenesis receptor-like kinases from Populus trichocarpa, Vitis vinifera, Daucus carota, Solatium tuberosum, Arabidopsis thaliana and Oryza saliva. HbSERKl contained a conserved intracellular serine/threonine protein kinase domain. After about 20 days of culture on the embryo induction medium, HbSERKl was expressed in calli, while HbSERKl was little expressed in the calli growing onto calli induction medium, leaf and latex. It was suggested that HbSERKl maybe take part in somatic embryogenesis during in vitro culture.关键词
巴西橡胶树/体细胞胚/类受体蛋白激酶/RT-PCRKey words
Hevea brasUieruis/ Somatic embryogenesis/ Receptor-like protein kinase/ RT-PCR分类
农业科技引用本文复制引用
王颖,陈洁,李辉亮,郭冬,彭世清..橡胶树体细胞胚胎发生相关类受体蛋白激酶基因[HbSERK1)的克隆及表达分析[J].热带作物学报,2011,32(8):1494-1498,5.基金项目
中央级公益性科研院所基本科研业务费专项资金资助项目(No.CATAS-ITBBKF093)和现代农业产业体系建设专项资金(No.CARS-34-GW1). （No.CATAS-ITBBKF093）
