热带作物学报2011，Vol.32Issue(8)：1499-1502,4.DOI:10.3969/j.issn.1000-2561.2011.08.023
巴西橡胶树病程相关蛋白基因(HbPR1)的克隆及分析
Cloning and Expression Analysis of Gene Encoding Pathogenesis-Related Protein1 in Hevea brasiliensis
摘要
Abstract
Based on the result from the sequence of PR1 in Arabidopsis thaliana and the data of RNA-Seq analysis in Hevea brasiliensis, the homolog of rubber PR1 was cloned by RT-PCR and named as HbPRl. The sequencing result showed that the open reading frame of HbPRl was 450 bp, encoding a 150 amino acids with a conserved SCP_PRl_like domain similar with PRls from other plants. Bioinformatics analysis showed that the amino acid sequence of the HbPRl was 76%, 74% and 72% similar with that of PRls from Populus tomentosa, Vita vinifera and Arabidopsis thaliana. The expression of HbPRl was induced by SA at transcript level. All the data indicated that HbPRl was the PR1 homolog and could be used as a gene marker for SAR in rubber.关键词
巴西橡胶树/系统获得性抗性/病程相关蛋白1(PR1)Key words
Hevea brasiliensis/ System acquired resistance (SAR)/ Pathogenesis-related protein 1 (PR1)分类
农业科技引用本文复制引用
罗红丽,秦云霞,闫志烨,向鹏..巴西橡胶树病程相关蛋白基因(HbPR1)的克隆及分析[J].热带作物学报,2011,32(8):1499-1502,4.基金项目
农业部橡胶树生物学重点实验室——海南省热带作物栽培生理学重点实验室开放课题项目(No.KLOF1101)和海南省自然科学基金项目(No.311030). （No.KLOF1101）
