热带作物学报2011，Vol.32Issue(9)：1708-1710,3.DOI:10.3969/j.issn.1000-2561.2011.09.025
芒果尖孢炭疽病病原茵的绿色荧光蛋白基因标记
Green Fluorescent Protein Transformed Colletotrichum acutatum from Mango
摘要
Abstract
Mango anthracnose is a major disease on mango (Mangifera indica L), caused by Colletotrichum gioeosporioides and(or)C. Acidatum. The protoplasts of a C. Acutatum the strain T7 were prepared by digesting the mycelia with lywallzyme. Furthermore, the expression vector pPCT74-sGFP was transformed into the protoplasts with PEG mediated method. Transformation were regenerated and selected on hygromycin-containing medium. Single-spore isolates obtained and showed strong green fluorescence after several successive generations on the medium without hygromycin. The results showed that pPCT74-sGFP was successfully integrated into the strain 17. The presence of the green fluorescent protein DNA in the fungal cells was confirmed by PCR using a GFP-specific primer pair. The transformed isolates did not differ markedly from the wild type isolate in growth and morphological characteristics in vitro. Pathogenicity tests showed that the transformation process did not alter virulence of the strain T7.关键词
芒果炭疽病/尖孢炭疽菌/绿色荧光蛋白基因/转化Key words
Mango anthracnose/ Colletotrichum acutatum/ Gfp gene/ Transformation分类
农业科技引用本文复制引用
张俊,吕延超,刘晓妹,张欣,张贺,蒲金基..芒果尖孢炭疽病病原茵的绿色荧光蛋白基因标记[J].热带作物学报,2011,32(9):1708-1710,3.基金项目
芒果病虫害综合防控及保鲜技术研究与示范(No.201203092-2) （No.201203092-2）
中国热带农业科学院环境与植物保护研究所基本业务费(No.2010hzsZDZX002). （No.2010hzsZDZX002）
