热带作物学报2011,Vol.32Issue(10):1853-1857,5.DOI:10.3969/j.issn.1000-2561.2011.10.013
辣椒MADS-box基因CaMADS的及表达分析
Cloning and Expression of CaMADS from Capsicum annuum
摘要
Abstract
The full-length cDNA encoding a MADS-box protein, designated CaMADS. Was isolated from Capsicum annuum. Sequence analysis showed that the length of CaMADS is 943 bp, it is consisted of a 744 bp open reading frame, a 25 bp 5'UTR and a 174 bp 3'UTR, encoding 247 amino acids with molecular weight of 28.7 Ku, isoelectric point(pI)of 9.32 and conservative MADS-box. The results of RT-PCR analysis indicated that CaMADS transcripts accumulated at relatively low levels in the flower and leaves. The expression of CaMADS increased during ripening of hot pepper, indicating that the CaMADS was associated with ripening of hot peppers fruit关键词
辣椒/MADS-box/表达Key words
Capsicum annuum/ MADS-box/ Expression分类
农业科技引用本文复制引用
孙继华,杨衍..辣椒MADS-box基因CaMADS的及表达分析[J].热带作物学报,2011,32(10):1853-1857,5.基金项目
中央级公益性科研院所基本科研业务费专项资金资助(No.Pzs05,Pzs064,Pzs065). (No.Pzs05,Pzs064,Pzs065)