热带作物学报2011，Vol.32Issue(10)：1878-1882,5.DOI:10.3969/j.issn.1000-2561.2011.10.018
胆木组织培养与快速繁殖技术研究
Tissue Culture and Rapid Propagation of Nauclea officinalis
摘要
Abstract
Tissue culture and rapid propagation of Nauclea officinalis were conducted with stem segments, leaves, and petioles of in vitro seedlings. Results demonstrated that the best treatment of seed surface sterilization was dipping in 70% alchol solution for 10 s, followed by dipping in 2% solution of the hypochlorite for 8-10 mim. The optima] media for multiple shoots induction and multiplication were 1/2 WPM + 0.5mg/L 2, 4-D, followed by 1/2 WPM+0.5 mg/L IBA and 1/2WPM+1.0 mg/L IBA+0.5 mg/L 6-BA. The multiplication coefficient and the germination rate of shoots was up to 4.8 and 94% respectively. The suitable media for shoot elongation and rooting were(l/2~l/4)WPM +0.5 mg/L 2, 4-D and(l/2-l/4)WPM+0.5 mg/L IBA. The survival rate of plantlets after acclimatization and transfer to glasshouse was approximately 80%.关键词
胆木/种子萌发/组织培养/快速繁殖Key words
Nauclea officinalis/ Seed germination/ Tissue culture/ Rapid propagation分类
农业科技引用本文复制引用
王和飞,张燕,林应邀,陈川平,刘进平..胆木组织培养与快速繁殖技术研究[J].热带作物学报,2011,32(10):1878-1882,5.基金项目
海南省教育厅高校科研项目"胆木微繁体系建立的研究"(No.Hjkj2010-44). （No.Hjkj2010-44）
