热带作物学报2011，Vol.32Issue(10)：1935-1939,5.DOI:10.3969/j.issn.1000-2561.2011.10.029
GFP标记的尖孢镰刀菌西瓜专化型侵染西瓜过程观察
Observation of the Infection Process of Watermelon by Fusarium oxysporum f.sp.niveum Using the GFP Marker
摘要
Abstract
Fusarium oxysporum f. Sp. Niveum(FON) was successfully transformed with the gene coding for green fluorescent protein (GFP). Transformants could emit quite stable fluorescence under 488 nm laser after four successive subcultures. PCR analysis confirmed that the gfp gene was transformed into FON. Observation of watermelon infection process by the GFP expressing FON strain was then carried out. Watermelon grown on 1/2 MS medium and soil were used for the study and their roots were inoculated with the FON strain. Results revealed that FON invaded into roots, pseudostem, and leaf vascular bundle after 48 hours, 3 days and 4 days, respectively, when the seedlings grown on the 1/2 MS medium were inoculated. On the soil-grown seedlings, FON was detected in roots, pseudostem and leaf vascular bundle after 2, 9 and 11 days, respectively. The infection of FON invading watermelon grown on the 1/2 MS medium is faster than in pots.关键词
尖孢镰刀菌西瓜专化型/GFP/西瓜/侵染过程Key words
Fusarium. Oxysporum f. Sp. Niveum/ Green fluorescent protein/ Watermelon/ Infection process分类
农业科技引用本文复制引用
李春强,梁慧施,夏亦荠,彭明..GFP标记的尖孢镰刀菌西瓜专化型侵染西瓜过程观察[J].热带作物学报,2011,32(10):1935-1939,5.基金项目
中央级公益性科研院所基本科研业务费(No.ITBBZX0821) （No.ITBBZX0821）
公益性行业(农业)科研专项(No.200903049) （农业）
海南省自然基金项目(No.310078). （No.310078）
