四川大学学报（自然科学版）2011，Vol.48Issue(6)：1397-1402,6.DOI:10.3969/j.issn.0490-6756.2011.06.031
麻疯树肌醇-1-磷酸合成酶基因的克隆与原核表达
Molecular cloning and prokaryotic expression of a novel 1L-myo-inositol-1-phosphate synthase gene from Jatropha curcas
摘要
Abstract
The lL-myo-inositol-1-phosphate synthase gene of Jatropha curcas was cloned by RACE tech nique. Its open reading frame is 1530 bp. Encoding 510 amino acids. Amino acid sequences analysis showed that JcMIPS had high similarity with MIPS genes of multiple plants, up 87. 08% to 91. 18%, and contained four conserved domains of the MIPS gene. The prokaryotic expression vector was con structed and was induced to express in 1 mmol/L IPTG and 18 ℃ for 5 hours to obtain a soluble protein. The purified target protein was identified by enzymatic reactions in vitro, suggesting that the sample had the enzyme activity and indicating the success of JcMIPS prokaryotic expression.关键词
麻疯树/肌醇-1-磷酸合成酶/克隆/原核表达Key words
Jatropha curcas/lL-myo-inositol-1-phosphate synthase/clone/prokaryotic expression分类
农业科技引用本文复制引用
喻川,苟春宝,黄静,王勇,陈放,魏炜..麻疯树肌醇-1-磷酸合成酶基因的克隆与原核表达[J].四川大学学报（自然科学版）,2011,48(6):1397-1402,6.基金项目
"十一五"科技支撑项目(2006BAD07A04) （2006BAD07A04）
四川省"十一五"支撑项目(2007BAD50B00) （2007BAD50B00）
