四川大学学报（自然科学版）2011，Vol.48Issue(6)：1456-1462,7.DOI:10.3969/j.issn.0490-6756.2011.06.041
大熊猫核糖体蛋白S15A亚基基因(rps15A)的克隆及序列分析
Cloning and sequence analysis of ribosomal protein S15A (rps15A) gene from the giant panda (Ailuropoda melanoleuca)
摘要
Abstract
The RT-PCR and Touchdown-PCR strategy were employed to isolate cDNA and genome of rpsl5A from the Giant Panda, respectively, sequenced and analyzed. The result showed that the cDNA fragment cloned was 460 bp length, containing an open reading frame(ORF)of 393 bp. The protein de duced was composed of 130 amino acids with an estimated molecular weight of 14. 84 kD and pi of 10. 62. The length of the genomic sequence is 6091 bp, which was found to possess 4 exons and 3 in trons. There are 5 different patterns of functional sites, one N-glycosylation site, one cAMP and cGMP dependent protein kinase phosphorylation sites, two protein kinase C phosphorylation sites, one Amida tion site,one Ribosomal protein S8 signature in the Giant Panda RPS15A protein. The deduced amino acid sequence of rpsl5A was analyzed and compared with human, other vertebrate, invertebrate of rpsl5A. The results showed that rps15A was highly similarity in eight other species: Homo sapiens, Bos Taurus, Pongo abelii, Rattus norvegicus , Mus musculus, Xenopus tropicalis, Danio rerio, Dro sophila melanogaster, suggesting RPS15A protein was conserved highly in evolution.关键词
大熊猫/rps15A/克隆/序列分析Key words
giant panda (Ailuropoda melanoleucg)/rpsl5A/cloning/sequences analysis分类
生物科学引用本文复制引用
李俊,丁祥,侯怡铃,孙冰,苏秀兰,吴光富,宋嫣,侯万儒..大熊猫核糖体蛋白S15A亚基基因(rps15A)的克隆及序列分析[J].四川大学学报（自然科学版）,2011,48(6):1456-1462,7.基金项目
国家自然科学基金(30470261) （30470261）
四川省应用基础项目(2009JY0061):四川省教育厅青年基金项目(092B088) （2009JY0061）
