四川林业科技2011，Vol.32Issue(4)：55-57,62,4.
桢楠DNA提取和RAPD条件的优化
DNA Extraction and Optimization of RAPD Reaction System for Phoebe zhennan
摘要
Abstract
DNA of Phoebe zhennan was extracted from the fresh leaves and silica gel dried leaves,and the various factors affecting RAPD reaction was optimized.The optimized reaction system and programme of RAPD for Phoebe zhennana were as follows：each 25 μL amplification reaction solution suited for Phoebe zhennana RAPD was consisted of 2μL DNA,0.3 μLTaq DNA polymerase,1 μL primer,5 μL MgCl2,0.5 μL dNTPs and 16.2 μL ddH2O.The PCR amplification program suited for Phoebe zhennan RAPD were pre-denaturing at 94 ℃ for 3 min,followed by denaturing at 94 ℃ for 1 min,annealing 36 ℃ for 1 min,extending 72 ℃ for 2 min and cycling 42 times,and lastly,extending 72 ℃ for 10 min and storing at 4 ℃.The results showed that same results of PCR amplification could be got from the fresh leaves and silica gel dried leaves and could meet the need for researches.关键词
桢楠/DNA提取/RAPD/体系优化Key words
Phoebe zhennan/DNA Extraction/RAPD/System optimization分类
农业科技引用本文复制引用
张炜,龙汉利,贾廷彬,谭斌,宋鹏,辜云杰,李晓清..桢楠DNA提取和RAPD条件的优化[J].四川林业科技,2011,32(4):55-57,62,4.基金项目
国家林业局林业公益性行业科研专项项目（项目编号：20110400104、200904044、201104109）共同资助 （）
