四川林业科技2011，Vol.32Issue(6)：73-75,3.
竹种内转录间隔区的核苷酸（ITS）序列分析
Nucleotide Sequences of the Internal Transcribed Spacers of Bamboo Species
摘要
Abstract
Using the fresh leaves of Neosinocalamus affinis,Dendrocalamus giganteus,Phyllostachys nigra as research materials,the extraction of DNA was done,and the genes of Internal Transcribed Spacer were amplified from genomic DNA by polymerase-chain-reaction（PCR）.Then the PCR products were sequenced and analyzed.The lengths of the PCR production were 390 bp,384 bp,and 380 bp for N.affinis,D.giganteus,and Phy.nigra respectively.The sequences are different to each other.Meantime,other ITS sequences of the genus in the NCBI were selected.Then the phylogenetic tree was established on the basis of the ITS sequences in order to analyze their evolutionary relationships.关键词
竹种/内转录间隔区（ITS）/序列分析/系统树Key words
Bamboo species/Internal Transcribed Spacer/Sequencing analysis/Phylogenetic tree分类
农业科技引用本文复制引用
杜健伟,于瑶,张汉尧..竹种内转录间隔区的核苷酸（ITS）序列分析[J].四川林业科技,2011,32(6):73-75,3.基金项目
西南生物多样性保育国家林业局重点实验室开放基金 （）
国家林业局918项目 （）
云南省重点学科（西南林业大学森林培育）资助 （）
