四川师范大学学报（自然科学版）2011，Vol.34Issue(6)：884-888,5.DOI:10.3969/j.issn.1001-8395.2011.06.023
粗毛栓菌atp9基因的克隆、表达及序列分析
Cloning, Expression and Sequence Analysis of the Subunit Gene Atp9 Unit in Trametes Gallica
摘要
Abstract
ATP synthase is a key enzyme of energy metabolism, which was involved in a variety of oxidative phosphorylation and photophosphorylation in vivo. The atp9 gene encoding the ninth part of ATP synthase A sub-unit is an important component of ATP synthase. It is closely related with respiration and photosynthesis. For its highly conserved sequence in evolution, one pair of primers were designed and synthesized according to the known gene sequences from closely related fungi. The first strand of Trametes gallica Cdna was amplified by reverse transcription and then the complete atp9 gene sequences were obtained by PCR. And then the product of PCR was ligated to the prokaryotic expression vector pET32a ( + ) and transformed into E. Coll for the expression of fusion protein. The sequenced and bioinformatics analysis showed that: the complete length of atp9 gene was 222 bp, and the peptide it encoded had 73 amino acids with a molecular mass of 7.35 kDa as predicted. Homology comparison and phylogenetic analysis indicated that the amino acid sequence of Trametes gallica atp9 gene possessed the highest homology with Crinipellis perniciosa and Trametes cingulata. This research laid the foundation for probing the function and metabolic pathways of atpA gene in the future.关键词
atp9基因/原核表达/序列分析/粗毛栓菌Key words
atp9 gene/prokaryotic expression/sequence analysis/Trametes gallica分类
生物科学引用本文复制引用
雍彬,司文杰,陶宗娅,严伟..粗毛栓菌atp9基因的克隆、表达及序列分析[J].四川师范大学学报（自然科学版）,2011,34(6):884-888,5.基金项目
四川省教育厅自然科学重点基金(10ZA006)资助项目 （10ZA006）
