水产学报2011，Vol.35Issue(11)：1684-1693,10.DOI:10.3724/SP.J.1231.2011.17563
大鳍鳠免疫球蛋白M重链基因的克隆及表达分析
Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus)
摘要
Abstract
Full cDNA sequence of a secretory IgM ( sIgM) gene in largefin longbarbel catfish (Mystus macropterus Bleeker) was cloned using RACE-PCR and RT-PCR methods. It has 1 992 nucleotides, including 5'UTR of 53 nucleotides, 3' UTR of 226 nucleotides and an open reading frame with 1 713 nucleotides encoding a 570 amino acid peptide. The deduced amino acid sequence in M. Macropterus contains 4 constant regions (CH) and a variable domain (VH) which consists of 4 frame regions (FRs)and 3 complementary determining regions (CDRs). The IgM comparison in seven teleost species showed that IgM in M. Macropterus shared the highest identity (54. 3% ) with that in Ictalurus punctatus, and the lowest identity (24.6% )with that in Epinephelus coioides, and that the conserved cysteines and tryptophans existed in all fishes involved. Phylogenetic tree based on some teleost Ig heavy chain amino acids suggested IgM in M. Macropterus was clustered closely with that of /. Punctatus. Fluorescent real-time PCR showed that IgM mRNA expression of M. Macropterus was mainly detected in blood cells, spleen, kidney and head kidney, and increased significantly in blood cells, spleen, head kidney between day 1 and day 10 after injection of Aeromonas hydrophila.关键词
大鳍鳠/IgM重链基因cDNA/克隆/实时荧光定量PCR/组织分布Key words
Mystus macropterus/ cDNA of immunoglobulin M heavy-chain gene/ clone/ real-time PCR/ tissue distribution分类
生物科学引用本文复制引用
李春涛,张其中,杨莹莹,朱成科,李超,陈霞..大鳍鳠免疫球蛋白M重链基因的克隆及表达分析[J].水产学报,2011,35(11):1684-1693,10.基金项目
国家"八六三"高技术研究发展计划(2011AA10A216) （2011AA10A216）
重庆市科技攻关计划项目(CSTC.1010AC1116) （CSTC.1010AC1116）
三峡库区生态环境教育部重点实验室基金资助项目 （）
