水产学报2011，Vol.35Issue(12)：1829-1836,8.DOI:10.3724/SP.J.1231.2011.17547
江蓠中类菌孢素氨基酸( MAAs)分离纯化及其成分分析
Study on the isolation, purification and composition analysis of mycosporine-like amino acids(MAAs) in Gracilaria changii
摘要
Abstract
Mycosporine-like amino acids(MAAs)found in marine organisms,especially in the algae were a class of ultraviolet absorption substances. To study the constituents and proportion of MAAs from Gracilaria changii of Zhanjiang, MAAs were separated and purified by C-18 Sep-Pak SPE and semi-preparative HPLC. Four mycosporine-like amino acids were found and were identified as palythine, shinorine, palythinol and porphyra-334,with an approximate ratio of 4.9:1:1. 2:9. 9,based on the characteristics UV absorption and spectral data. This paper compared the accuracy of UV and HPLC determination for the content of porphyra-334 which was the main component of G. Changii,and established fitting curves of two methods. Verified by experiment, the HPLC detection for the main components of MAAs was ultimately determined. This research laid the foundation for the development and use of the UV radiation and anti-oxidative substances in the economic algae produced in our country.关键词
江蓠/类菌孢素氨基酸/ESI-TOF/MS/检测方法Key words
Gracilaria changii/ mycosporine-like amino acids/ ESI-TOF/MS/ detection分类
生物科学引用本文复制引用
金宁宁,张朝辉,李八方,严芳芳,孙京沙..江蓠中类菌孢素氨基酸( MAAs)分离纯化及其成分分析[J].水产学报,2011,35(12):1829-1836,8.基金项目
国家自然科学基金项目(30771674) （30771674）
教育部重点项目(109099) （109099）
