四川医学2012，Vol.33Issue(1)：12-14,3.
流式细胞术检测O型孕妇血液中胎儿微量A(B)红细胞与新生儿溶血病发病率的相关性研究
The correlation studies on the quantities of the fetal RBCs in the pregnancies with type O be quantified by flow cytometer (FCM) and the incidence of hemolytic disease of newborn( HDN)
摘要
Abstract
Objective To use the noninvasive method of flow cytometer( FCM) detecting the hemolytic disease of new-bom (HDN) due to the mismatching of ABO blood group system, exploring the practical of this method. Methods Using the indirect method(IgG anti-A(B) as the first antibody,FITC- anti- IgG F(ab')2 as the second antibody) to dyeing the cells,and the trace quantities of the fetal RBCs in the pregnancies with type 0 was quantified by flow cytometer ( FCM). Results Difference between the two methods of flow cytometer( FCM) and serological test was significant . The detected data in the investigation shows that it could be used to prevent hemolytic disease of newborn(HDN) ,if the ante-natal detecting be carried out much earlier.Conclusion Establishing the noninvasive method of flow cytometer( FCM) to detect the hemolytic disease of newborn (HDN). It may be used for diagnosis of hemolytic disease of newbom(HDN)due to the mismatching of ABO blood group system in ante-natal detecting.关键词
流式细胞术/ABO血型/新生儿溶血病/非侵入性检测Key words
flow cytometer(FCM) /ABO blood group system/ hemolytic disease of newborn (HDN) / noninvasively detecting分类
医药卫生引用本文复制引用
周英,吕文彬,李健,陈雪,王乃红,迭敏,方军..流式细胞术检测O型孕妇血液中胎儿微量A(B)红细胞与新生儿溶血病发病率的相关性研究[J].四川医学,2012,33(1):12-14,3.基金项目
2008年四川省卫生厅科研课题(编号:080085) （编号:080085）
