山东科学2011，Vol.24Issue(6)：36-38,61,4.
pH区带精制逆流色谱法制备青风藤碱
Preparation of sinoacutine from Stephania kwansiensis by pH-zone-refining counter-current chromatography
摘要
Abstract
We employed pH-zone refining counter-current chromatography to prepare high-purity sinoacutine from Stephania kwangsiensis. Its solvent system was composed of petroleum ether-ethyl acetate-methanol-water （3 ： 7： 1 ： 9, V/V）, in which triethylamine （ 10 mmol/L） was added to the upper organic stationary phase as a retainer and hydrochloric acid （10 mmol/L） was added to the aqueous mobile phase as an eluter. The structure of sinoautine was identified by ESI-MS,1H NMR and 13C NMR. The purity of sinoacutine determined by RP-HPLC was 99.5%. This approach has such advantages as high-efficiency and high-purity, suitable for the preparation of large amount of sinoacutine.关键词
广西地不容/青风藤碱/pH区带精制逆流色谱法Key words
Stephania kwangsiensis/Sinoacutine/pH-zone-refining counter-current chromatography分类
医药卫生引用本文复制引用
董红敬,张永清,庄会永,李佳,耿岩玲,王晓..pH区带精制逆流色谱法制备青风藤碱[J].山东科学,2011,24(6):36-38,61,4.基金项目
重大新药创制项目 （）
山东省科技攻关项目 （）
济南市高校院所自主创新计划 （）
山东省中青年科学家科研奖励基金 （）
