山东科学2011，Vol.24Issue(6)：39-43,5.
超临界CO2萃取-高速逆流色谱分离纯化欧前胡素和异欧前胡素
Separation and purification of imperatorin and isoimperatorin with supercritical CO2 fluid extraction- high-speed counter-current chromatography
摘要
Abstract
This paper addressed the extraction of imperatorin and isoimperatorin from Angelica dahurica （Fisch. ex Hoffm. ） with supercritJcal CO2 extraction method. Orthogonal experiments show that the optimal technological conditions of this extraction method are temperature of 60 ℃, pressure of 2.5 MPa, sample particle size of 40 -60 mesh and 50 times amplification of a preparative supercritical fluid extraction （SFE） system. We separated and purified imperatorin and isoimperatorin with high-speed counter-current chromatography and a two-phase solvent system of 1 ： 0.4： 1. 1： 0.5 （ n- hexane-ethyl vs. acetate vs. methanol vs. water, V/V）. We also employed HPLC-DAD and MS to perform quantative and qualitive analysis for imperatorin and isoimperatorin. The purities of them were both above 99.0%. The method is convenient and fast,with a high reproducibility.关键词
超临界CO2萃取/高速逆流色谱/欧前胡素/异欧前胡素Key words
supercritical CO2 fluid extraction/HSCCC/imperatorin/isoimperatorin分类
医药卫生引用本文复制引用
林小静,耿岩玲,刘峰,傅茂润,刘建华,王晓..超临界CO2萃取-高速逆流色谱分离纯化欧前胡素和异欧前胡素[J].山东科学,2011,24(6):39-43,5.基金项目
济南市留学人员创业计划 （）
济南市高等院校自主创新计划 （）
山东省科学院科技发展基金项目（科基合字（2010）第18号） （）
