山东医药2011，Vol.51Issue(50)：1-3,3.
拉帕替尼诱导Her-2高表达乳腺癌细胞凋亡的机制探讨
Mechanism of Her-2 overexpressed breast cancer cell apoptosis induced by lapatinib
摘要
Abstract
Objective To investigate the mechanism of breast cancer SKBR-3 cells apoptosis induced by lapatinib. Methods SKBR-3 cells in log phase were cultured with 96 well plate,and divided to experiment group and control group randomly, 6 wells each. Cells in experiment group was treated with 500 μg/L lapatinib and the control group was treated with DMSO and DMEM for 24,48,72 h. The MTT assay was used to detected the SKBR-3 cells survival rate, the flow cy-tometry was used to detect the cell apoptosis ,and Western blot assay was used to detectd the expression of P-AKT and sur-vivin. Results The survival rate of SKBR-3 cells cultured with lapatinib for 24,48,72 h were 100% ,69.4% ,62.0% (P <0.05). Compared with control group, the apoptotic rate in experiment group and control group were 10. 48% and 2. 14%(P<0.05).The relative expression level of P-AKT and survivin were 0.49 ±0. 12 and 0.51 ±0.38 ,but 1.46 ± 0. 13 and 0.89 ±0.36 in control group( P <0.05). Conclusion p-AKT and survivin play important roles in the apoptosis of SKBR-3 cells induced by lapatinib.关键词
人表皮生长因子受体2/乳腺癌/拉帕替尼/细胞凋亡/磷酸化蛋白激酶B/生存素Key words
human epidermal growth factor receptor2/ breast cancer/ lapatinib/cell apoptosis/ phosphorylated protein kinase B/ survivin分类
医药卫生引用本文复制引用
张红,毛晓韵,林哲洙,房月,金锋..拉帕替尼诱导Her-2高表达乳腺癌细胞凋亡的机制探讨[J].山东医药,2011,51(50):1-3,3.基金项目
国家自然科学基金资助项目(30950009) （30950009）
辽宁省科技厅博士科研启动基金项目(20091110). （20091110）
