摘要
Abstract
Objective To exploe the effect of vitamin A on cervical cancer cell proliferation and apoptosis. Methods The proliferation inhibition rate of human cervical cancer Caski was analyzed by MTT after treating by 5, 7. 5, 10, 15 μg/ml of vitamin A. Cell cycle of Caski, Siha and Hela treated by vitamin a were detected by flow cytometry. Mitochondri-al membrane potential was examined by fluorescence microplate reader; Apoptosis of Caski cell treated with vitamin A was detected by deoxynucleotidyl TUNEL. Results Cell proliferation inhibition rates of Caski treated by 5,7.5,10,15 μg/ml vitamin A for 24 h were 32. 1% ±2.6%, 47.3% ±1.1%, 66.4% ±2.1%, 70.8% ±2.7%, respectively, after 48 h treatment were 38.3% ±2.8% , 52.6% ±3. 1% , 72.5% ±2.4% , 80.0% ±4.9%. Vitamin A can inhibit cervical cell proliferation in a time and concentration dependent manner (all P < 0.05). The number of Caski, Siha and Hela cells after vitamin A treatment in G0/G1 and G2/M phase significantly reduced, while S phase cells increased, cells were blocked in the Go phase, the mitochondria potential of Caski, Siha and Hela cells was 2. 21 ±0. 29, 2. 05 ±0. 35, 2. 38 ±0. 22, respectively, and pre-treatment was 0. 32 ± 0.08, 0. 24 ± 0. 15, 0.41 ± 0. 06 (all P < 0. 05). Conclusions Vitamin A can inhibit cervical cancer cell proliferation and induced apoptosis.关键词
维生素A/宫颈肿瘤/宫颈癌/细胞凋亡Key words
vitamine A/ cervical neoplasm/ cervical cancer/ apoptosis分类
医药卫生
