山东医药2011，Vol.51Issue(51)：7-9,3.
BIM重组腺病毒转染对EGFR突变NSCLC细胞凋亡及BIM表达水平的影响
Effect of recombinant BIM on apoptosis and BIM expression transfection in NSCLC with EGFR mutation
摘要
Abstract
Objective To analyze the effect and the mechanism of the apoptosis in non-small cell lung cancer cell (NSCLC) transfected by recombinant BIM. Methods A549, H1650, H1975 in logarithmic growth phase were divided into three groups randomly, transfect group was transfected by recombinant BIM, negative control group ( NC group) was transfected without recombinant BIM, black control group ( BC group) was not transfected. After 48 h, apoptosis of cell strains transfected by recombinant BIM was measured by Annexin PI; expression of BIM transfected by recombinant BIM was detected by western blot assay. Results In contrast with NC and BC groups, the apoptosis rate and the expression level of transfect group increased observably, and which were higher in the H1650 and H1975 mutant cell lines than those in the A549 cells (P <0. 01). Conclusion Recombinant BIM transfection can induce the apoptosis in NSCLC cells with EGFR mutations, which possibly caused by increasing the expression of BIM; this provides basis for the gene therapy of NSCLC.关键词
促凋亡蛋白/转染/非小细胞肺癌/细胞凋亡Key words
BIM/ transfection/ non-small cell lung cancer/ apoptosis分类
医药卫生引用本文复制引用
庞龙滨,贺韦东,邢春燕,王曙光,邓亚妮..BIM重组腺病毒转染对EGFR突变NSCLC细胞凋亡及BIM表达水平的影响[J].山东医药,2011,51(51):7-9,3.基金项目
山东省自然科学基金资助项目(Q2008C05). （Q2008C05）
