山东医药2011，Vol.51Issue(52)：4-6,3.
雌激素对雌激素受体阴性的子宫内膜腺癌细胞系JEC的作用
Effects of estrogen on growth in estrogen receptor-negative endometrial adenocarcinoma cell line JEC
摘要
Abstract
Objective To study the effects of different concentration of estrogen ( E2 ) on the growth of estrogen recep tor-negative endometrial adenocarcinoraa cell line JEC in vitro. Methods JEC was cultured in vitro. Experimental groups ( E2 of different concentrations ) and control group ( without E2 ) were set up in the test. The proliferation of JEC was valua ted by MTT. The cell cycle was analyzed by flow cytometry. The change of Ca2+ in JEC was examined by laser scanning confocal microscope. Results The optical density (OD) of JEC in 10 -7, 10 -8 and 10-9 mol/L concentration of E2 at the 4th and 5th days was higher than those in control group (P<0. 05). OD of JEC in 10 mol/L concentration of E2 at the 5th day was higher than that in control group (P<0. 01). The cell cyclic distribution of JEC was unchanged than those in control group in the 4th and 5th days after dealing with 10 mol/L E2. The average fluorescence intensity of intracellular Ca2+ after dealing with 10-7 mol/L E2 was higher than that in control group (P<0.01). Conclusions Estrogen can stim ulate the proliferation of estrogen receptor-negative JEC in vitro. It may be associated with the enhance of Ca2 + .关键词
子宫内膜肿瘤/受体,雌激素/细胞周期Key words
endometrial neoplasms/ receptors, estrogen/ cell cycle分类
医药卫生引用本文复制引用
王美丽,吴中明,王欢,王玉..雌激素对雌激素受体阴性的子宫内膜腺癌细胞系JEC的作用[J].山东医药,2011,51(52):4-6,3.基金项目
贵州省优秀科技教育人才省长专项资金项目[黔省专合字(2005)313号]. （2005）
